News for April 10, 1997

Sorensen will ride Paris-Roubaix

Sorensen, the current World Cup series leader, had originally said he would miss cycling's premier one day race.

"Rolf has changed his mind because he is in such good form," Theo de Rooy, his Rabobank team chief, said.

"Paris-Roubaix is such an historic race and he has a lead to maintain. He has a good opportunity to finish high up and score good points," De Rooy added.

Sorensen, currently riding in the Tour of the Basque country, will ride the following week in Luik-Bastenaken-Luik.

There are ten Dutch riders on the rider's list for the third World Cup Race next weekend. The race over 262 kilometers in the north of France will have from Rabobank Sorensen, Aart Vierhouten, Max van Heeswijk, Leon van Bon, Jans Koerts, Erik Dekker and the foreign riders Piziks and McEwen. TVM will enter Tristan Hoffman, Steven de Jongh, Servais Knaven, Louis de Koning en de buitenlanders Johan Capiot, Hendrik van Dijck, Peter van Petegem and Jesper Skibby.

Lance Update

BLOOD TESTING FOR PROFESSIONAL CYCLISTS: What's a fair hematocrit limit?

David T. Martin , Michael Ashenden, Robin Parisotto, David Pyne, Allan G. Hahn, Department of Physiology and Applied Nutrition, Australian Institute of Sport, Belconnen, Australia

The decision of the UCI to use blood tests to deter the use of rhEPO by professional cyclists is admirable, but the 50% hematocrit limit may be too strict. Ten years of data collected from road cyclists at the Australian Institute of Sport indicate that a 52% hematocrit limit would result in fewer false positive tests.

Attempts to Discourage Use of rhEPO

The news has made headlines on sport pages around the world. Both the Federation Internationale de Ski (FIS) and the Union Cycliste Internationale (UCI) have implemented random blood tests prior to competition to deter the alleged use of DNA-recombinant human erythropoetin (rhEPO), a hormone known to increase the rate of red blood cell production. These decisions have come after years of finger-pointing and unsubstantiated claims that some of the world's most competitive cyclists and cross-country skiers are receiving an illegal performance advantage by injecting rhEPO. The governing bodies for these two endurance sports have decided upon slightly different methods and cut-off values for screening blood samples. Whereas FIS have identified specific hemoglobin concentrations (16.5 g/dl women; 18.5 g/dl men), the UCI have decided to use a 50% hematocrit for male cyclists.

Seiler (1997) has recently reported on blood testing cross-country skiers to deter the use of rhEPO. This report will focus on similar issues faced by the international cycling community.

No More Hematocrits Higher Than 50%

At their annual meeting in Geneva (24 January 1997), UCI delegates, in consultation with medical professionals, decided to implement blood testing to deter the alleged use of rhEPO by selecting a 50% hematocrit as an upper limit. In an article entitled "Cycling Pro's Saddled with Blood Tests" appearing in The European (30 Jan - 5 Feb, 1997), it was reported that random blood tests would begin on March 22 at the Milan to San Remo Classic bike race. UCI president, Hein Verbruggen, has stressed that the testing is a " health check" and that a positive test does not imply rhEPO use. The testing has been primarily established to insure that professional cyclists will not begin a major road race with a dangerously high hematocrit. In contrast to a positive drug test, which can result in prolonged suspension from competition, riders with a hematocrit greater than 50% would be suspended only until values decreased to an acceptable level. But are there data indicating that a 51% hematocrit is dangerous for a professional road cyclist? And more importantly, what is the normal incidence of a hematocrit above 50% in professional cyclists not taking rhEPO? It appears that answers to these questions are currently not available.

RhEPO Can Be Dangerous

It is now well recognized that the uncontrolled use of rhEPO can be dangerous. Within the first four years of rhEPO's introduction, this synthetic hormone was suggested to have caused over 17 athlete deaths (Ramotar, 1990). Some news reports are now suggesting that over 20 competitive cyclists have died as a result of rhEPO administration (The European, 12-18 Dec, 1996). Never-the-less, international competition is fierce and many competitive cyclists desire the financial gains and prestige associated with being a champion. Perhaps this is why the suspected incidence of rhEPO use among professional cyclists in high. An article presented in The European (5-11 Dec, 1996) has summarized a controversial dossier prepared by Sandro Donati, an Italian professor of exercise physiology. Donati's reports suggests that, "rhEPO was being used by 60-70% percent of all professional cyclists." Additionally, the dossier presents the names of 21 professional riders including three well known cyclists. Donati also names seven Italian sport doctors who supposedly administered rhEPO to road cyclists for large annual fees ($50,000-$100,000). Some of those implicated have adamantly denied allegations indicating that there is no proof to support the outlandish claims.

Why a 50% Hematocrit?

Although the UCI should be congratulated for their efforts to prevent the illegal use of rhEPO, the 50% hematocrit limit (approximately 16.5 g/dl) may be too aggressive and result in many false positive tests. The UCI cut-off is obviously much lower than the 18 g/dl hemoglobin limit selected by FIS. In response to a query made by the Department of Physiology and Applied Nutrition at the Australian Institute of Sport, the UCI explained that the 50% cut-off valued was agreed to by sport doctors and team directors working for the major professional cycling teams. The UCI explained that, a 53% hematocrit limit was initially proposed (based on three standard deviations above the average hematocrit value for a normal population) but then decreased to 50% to ensure that all professional team delegates were in agreement. Why would doctors with professional cycling teams push for a lower detection limit? Maybe "clean" cycling programs want to insure that drug users will be caught or at least be more limited with their illegal activities. Or perhaps there is evidence indicating that a 51% hematocrit is dangerous for endurance athletes.

Hematocrits in Australian Road Cyclists

Unfortunately, the prevalence of a hematocrit greater than 50% in elite road cyclists not taking rhEPO is not well established. Additionally, the effects of body posture, dehydration and altitude training on the hematocrit of professional cyclists is not well documented. At the Australian Institute of Sport , top Australian Road Cyclists (including five Professional Australian Cyclists who are now racing in Europe) have participated in routine blood tests to monitor health and training stress. As part of the normal venous blood analysis, hematocrit is determined using routine laboratory equipment (a Coulter Counter). A retrospective analysis of 360 blood samples collected from Australia's top road cyclists between 1987-1996 resulted in 10 hematocrit values of 50% or more. Thus, 2.8% of blood samples from the Australian Road Cyclists exceed the 50% hematocrit value recently set by the UCI as an acceptable limit. It is believed that these cyclists were training and competing in a "clean environment" at the time of testing. In other words, it is unlikely that they were taking rhEPO. The maximum hematocrit 52.0%, recorded in one cyclist.

Hematocrits in Other Competitive Australian Athletes

In a much larger data set (12,359 blood samples) that including male and female athletes participating in a variety of sports (e.g., swimming, athletics, water polo, weight lifting, rowing, tennis, soccer, basketball, netball, gymnastics) 3.4% of blood samples had a hematocrit value greater than 50%. The percentage of hematocrit scores greater than 50% is therefore similar between the Australian cyclists and other Australian athletes competitive at the national level. A more detailed examination of the hematocrit data from all sports determined that the percentage of tests where hematocrit was greater than 50% ranged from 0.3% in netball to 25.7% in weight lifting.

Effects of Posture on Hematocrit

Hematocrit reflects the balance between red blood cell production and destruction but is also known to be influenced by dehydration and body posture. Heart rate and blood pressure are higher when standing compared to sitting or lying down. The higher blood pressure when standing is associated with the movement of intravascular fluid (i.e., plasma) into interstitial compartments. This causes plasma volume to decrease and hematocrit to rise. Gore and Colleagues (1992) have reported a 6% decrease in plasma volume associated with 30 minutes of standing following 30 minutes of sitting. The following example illustrates how body position could determine whether a cyclist is disqualified or not based on the 50% hematocrit UCI ruling. If a cyclist has a blood volume of 6.0 liters and a hematocrit when sitting of 49%, then the blood would be composed of 2.94 liters of red blood cells and 3.06 liters of plasma. If the cyclist stands for 30 minutes and undergoes a 6% reduction in plasma volume, hematocrit would now rise to 50.5% and under the new UCI ruling the cyclist would be disqualified. It is worth noting, that at the Australian Institute of Sport venipucture blood draws were performed on athletes in the morning in a fasted state after remaining in a supine position for 5-10 minutes. If blood was collected from the Australian cyclists in a seated or standing position, it is likely that more than 2.8% of the blood samples would have a hematocrit greater than 50%. In attempts to control for extraneous variables such as posture, the UCI has established that blood will be taken from cyclists in the morning, before breakfast, in a seated position.

Direct Detection of rhEPO

Techniques have been developed to test for rhEPO use but thus far, none have proven to be completely satisfactory for screening athletes. Wide et al. (1995) have demonstrated that rhEPO can be distinguished from endogenous EPO in both urine and serum samples using electrophoretic techniques. The electrophoretic technique is based on the observation that endogenous EPO generally has a greater negative charge than rhEPO. Although no false positive tests are detected using this technique, it appears that the use of rhEPO can only be confirmed up to three days after injections have stopped. The success of this technique would therefore depend upon random "out-of-competition" sample collections.

Indirect Detection of rhEPO

Another potential method for determining whether an athlete is using rhEPO has been suggested by Casoni et al., (1993). Using sophisticated scattering laser technology Bayer has introduced a series of hematology analyzers that allow for red blood cell volume and hemoglobin content to be evaluated on a cell by cell basis. The Technicon Bayer H*1, H*2, and H*3 analyzers allow for the distribution of cell size to be plotted against cell hemoglobin content resulting in a nine quadrant cytogram. Casoni and colleagues (1993) observed that the percentage of cells that were macrocytic (>120fl) and hypochromic (<28 pg) was greater than 0.6% only in those subjects who underwent rhEPO therapy. A limitation to this technique was is that more than 0.6% macrocytic-hypochromic cells could only be observed towards the end of, and for 10 days following a 45 day course of rhEPO. Also, approximately 60% of rhEPO treated subjects tested below the 0.6% cut-off value.

Recently, Bayer Health Care Australia has entered into a sponsorship agreement with the Sports Haematology and Biochemistry Laboratory at the Australian Institute of Sport. This sponsorship has resulted in the acquisition of a Technicon H*3 analyzer which is currently being used to analyze athletes' blood. A retrospective analysis of blood samples provided by 50 male endurance athletes (8 swimmers, 12 cyclists, 30 rowers) was performed to evalute the incidence of macrocytic-hypochromic red blood cells. In all but one sample the percentage of macrocytic hypochromic cells was equal to or less than 0.2%. The highest value measured in this group was only 0.3%. It should be noted that all of these athletes were tested during the initial phases of a competitive season in the summer. As mentioned by Wide and co-workers (1993), a combination of direct and indirect techniques will likely emerge in the near future to enable rhEPO use to be confidently detected.

In Conclusion

The decision of the UCI to test the blood of professional cyclists to deter the use of rhEPO is admirable. However, the 50% hematocrit limit appears too aggressive on the basis of 10 years of data collected from road cyclists tested at the Australian Institute of Sport. A 52% hematocrit limit would result in fewer false positive tests and could still deter the suspected use of rhEPO. While elite athletes wait for sensitive rhEPO detection techniques to emerge, measurements of hematocrit may represent a temporary deterrent. However, prior to implementing blood testing for hematocrit it would seem worth while to carefully evaluate the cut-off level and also consider the influence of body position, altitude training and dehydration on hematocrit values in professional cyclists. It is now possible that the leader in the Tour de France could be prevented from competing in the final stage because his hematocrit is "dangerously" high. With lucrative salaries at stake, it will be interesting to see if the 50% hematocrit rule will last throughout the 1997 professional cycling season.

References

Martin, D. T., Ashenden, M., Parisotto, R., Pyne, D., Hahn, A. G. (1997). Blood testing for professional cyclists: what's a fair hematocrit limit? Sportscience News, (Mar-Apr), http://www.sportsci.org/news/news9703/AISblood.html

Casoni I., Ricci G., Ballarin E., et al. (1993). Hematological indices of erythropoietin administration in athletes. International Journal of Sports Medicine, 14, 307-311.

Gore C., Scroop G., Marker J., Catcheside P. (1992). Plasma volume, osmolarity, total protein and electrolytes during treadmill running and cycle ergometer exercise. European Journal of Applied Physiology and Occupational Physiology, 65, 302-310.

Ramotar J. (1990). Cyclists' deaths linked to erythropoietin? Physician and Sports Medicine, 18, 48-49.

Seiler, S. (1997). Tighter control on EPO use by skiers. Sportscience News, (Jan-Feb), http://www.sportsci.org/news/news9701/EPOfeat.html

Wide L., Bengtsson C., Berglund B., and Ekblom B. (1995). Detection in blood and urine of recombinant erythropoetin administered to healthy men. Medicine and Science in Sports and Exercise, 27, 1569-1576.

Sorensen late at Start

And a trifle from the Ronde

And a little fact from the Ronde van Vlaanderen: who was the last rider who signed on the riders list before the start in St Niklaas?

Rolf Sorensen....

Roger who was over the channel in belgium for the weekend writes:

Yes, this is right. The entrance into the route to the platform for signing on was pretty well blocked off by spectators and barriers by the time Sorensen arrived about two minutes before the fictive start at 9.55. One of my trip companions says Sorensen was receiving strong instructions from the team boss in the middle of the square. He happened to have to pass through the barrier just where I was heading away from them. "Excuse me," he said in English as he wove his way through.

GP Clermontois (France)

5.4.97

1. Stage Road Race

 1. Catherine Marsal
 2. Samokhvalova (RUS)	
 3. Rouleau (FRA)	
 4. Els Decottenier (BEL)
 5. Evi Gensheimer

6.4.97 

2. Stage 7km ITT

 1. Catherine Marsal
 2. Samokhvalova (RUS) 30sec.	
 3. Evi Gensheimer (GER) 34sec.

6.4.97 

3. Stage - Road Race

 1. Evi Gensheimer (GER)

News from Austrial

Georg Totschnig (No. 78) will not start in Giro, because TELEKOM will start only the Tour de Franc.

But what are the chances for a start of Georg Totschnig (last year place 6 in VUELTA):

TELEKOM will start with the captain Riis, Jan Ulrich is 2nd captain,if B.Riis will get problems. Udo Boelts is a one of the helpers of Riis, Erik Zabel (defending the green jersey) will get two helpers for sprint - only two other cyclist will have a chance to start - Hundertmarck and Totschnig ?

TOUR OF AUSTRIA:

29.5.97 - 7.6.97

 29.5. Prolog Wien - Praterstraße   3 km
 30.5. Eisenstadt - Graz          196 km
 31.5. Graz - Gröbming            208 km
  1.6. Gröbming - Trumersee       160 km
  2.6. Trumersee - Bad Hofgastein 160 km
  3.6. Bad Hofgastein über den
       Großglockner nach Lienz    148 km
  4.6. Lienz - St.Johann/Pongau   153 km
  5.6. St.Johann - Villach        170 km
  6.6. Faakersee WM-Kurs          168 km
  7.6. Villach - A1-Ring Austria  173 km

 1.539 km

 Mountains:

 Hors Kategorie
 Fuschertörl      2.428 m     Stage 5 ("Grossglockner")

 Kategorie I
 Sölkpaß          1.788 m     Stage 2
 Tauernpaß        1.739 m     Stage 7

 Kategorie II
 Gaberl           1.547 m     Stage 2
 Hochtor          2.575 m     Stage 5
 Felbertauernpaß  1.511 m     Stage 6
 Katschberg       1.641 m     Stage 7 

Vandenbroucke and T. Steels have signed, no Austrian pofessional will start.